FastQCFastQC Report
Mon 6 Feb 2012
7.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename 7.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10561796
Filtered Sequences 0
Sequence length 50
%GC 47

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC 2252367 21.325605985951633 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC 933962 8.842833169661676 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG 740153 7.007832758746712 TruSeq Adapter, Index 8 (100% over 49bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT 252295 2.3887509283458987 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG 239209 2.2648515460817458 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCACCATTTGAAATCAGTGTTAGATCGGAAGAGCACACGTCTGAACTC 107601 1.0187755946053114 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA 90767 0.8593898234732047 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA 84874 0.8035943886816219 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 71376 0.675794154706264 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT 70069 0.663419365418533 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC 65276 0.6180388259724009 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC 62357 0.5904014809602458 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT 55370 0.5242479593432783 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACAGTGGCTAAGTTCCGTAGATCGGAAGAGCACACGTCTGAACTCCA 45304 0.42894219884572665 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGAAAGATCGGAAGAGCACACGTCTGAACTC 44969 0.42577038980870296 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGT 41664 0.3944783633389624 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT 41305 0.3910793202216744 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC 40024 0.3789507011875632 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC 38194 0.36162410256740424 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA 37984 0.3596358043650909 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTAGATCGGAAGAGCACACGTCTGAACTCCAG 37444 0.35452303755914244 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC 28995 0.27452717321940323 TruSeq Adapter, Index 8 (100% over 50bp)
CAGAGTTCTACAGTCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC 27472 0.26010727720929283 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC 25226 0.23884195453121795 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGCAGATCGGAAGAGCACACGTCTGAACTCCA 24551 0.2324509960237823 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA 22817 0.2160333337246809 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG 22462 0.21267216295410363 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA 22192 0.21011577955112937 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT 21543 0.20397099129731344 TruSeq Adapter, Index 8 (100% over 48bp)
TAGCCTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC 21231 0.2010169482538765 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG 18927 0.1792024765484961 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCNTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 18723 0.1772709868662489 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC 18661 0.17668396549223256 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC 15938 0.1509023654689032 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTAGATCGGAAGAGCACACGTCTGAACTCCA 14964 0.14168044904484048 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGTTCTACAGTCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA 14433 0.13665289501899108 Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCCAG 13750 0.13018619181813396 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCCTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 13473 0.12756353180841593 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TAGCTTATCAGACTGATGTAGATCGGAAGAGCACACGTCTGAACTCCAGT 13178 0.12477044623849959 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGAACTGAATTCCATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC 13178 0.12477044623849959 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCCTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC 12999 0.12307565872319443 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC 12875 0.1219016159751618 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCGTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 12822 0.12139980738124463 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGAGAACTGAATTCCATAGGCTGTAGATCGGAAGAGCACACGTCTGAACT 12707 0.12031097741331115 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA 12433 0.11771672166362615 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGACACAATTGAATCTCGTATG 12090 0.11446916793318106 Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC 11894 0.1126134229443553 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTATAAAGCAATGAGACTGATTAGATCGGAAGAGCACACGTCTGAACTCC 11874 0.11242406121080165 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCGTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC 11313 0.10711246458462177 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGAGATCGGAAGAGCACACGTCTGAACTCCAG 11139 0.10546501750270502 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC 10861 0.10283288940630932 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CGTCT 9910485 31.20691 463.26022 40
GTCTG 9961745 29.863573 441.8914 41
TCTGA 10073095 24.772514 361.67297 42
CACGT 9924465 22.99319 339.45325 38
ACGTC 9913580 22.967974 339.93524 39
GATCG 10204290 22.507404 328.22137 25
ATCGG 10164870 22.420458 328.608 26
TCGGA 10088690 22.25243 318.72134 27
CACAC 11183835 20.024824 245.19633 36
AGATC 10162715 18.388796 268.1286 24
CTGAA 10015220 18.121912 266.39816 43
GAACT 9891055 17.897243 265.60822 45
TGAAC 9810885 17.75218 265.96936 44
AACTC 9154520 17.399174 278.67438 46
ACACG 9898570 16.873335 250.26613 37
AGCAC 9881255 16.843819 232.22664 34
GCACA 9707025 16.546824 231.84134 35
CGGAA 10035950 16.286863 234.36232 28
AGAGC 9828235 15.949773 222.43802 32
GAGCA 9818480 15.933941 222.00116 33
GGAAG 10204955 15.766691 223.93105 29
TGTTG 4833095 15.37966 675.7011 17
GAAGA 11764325 14.910676 183.6139 30
ACTCC 5925875 14.4209585 206.59639 46
AAGAG 10047525 12.73472 183.11548 31
ATGTT 4868315 12.708694 558.4378 16
TTATC 4571645 12.535575 562.4808 5
CTGAT 5026160 12.360713 528.8087 13
CTTAT 4470125 12.257205 560.69293 4
TGATG 4964680 11.623821 503.04196 14
GATGT 4925395 11.531843 503.3956 15
GTTGA 4893175 11.456408 495.10236 18
GACTG 5071070 11.185161 474.99902 11
GCTTA 4455450 10.95718 501.4132 3
TAGCT 4446905 10.936164 499.7447 1
AGCTT 4419700 10.86926 500.60623 2
TCAGA 5563575 10.066938 392.1189 8
ATCAG 5484970 9.924709 389.52322 7
CTCCA 4056350 9.871362 113.38092 25
TATCA 4842675 9.769975 433.42233 6
ACTGA 5086195 9.20315 388.29913 12
AGACT 4968540 8.990261 388.57846 10
TTGAC 3467590 8.527759 367.5966 19
CAGAC 4977640 8.4850025 366.14856 9
TCCAG 3381230 7.8336987 107.88504 26
CAGAT 4035385 7.301776 236.83415 23
CAGTC 2898560 6.71544 99.10578 28
CCAGT 2623585 6.078372 99.18828 27
TGACA 3339520 6.042651 260.30765 20
TTGAA 2870965 5.5142503 107.863205 19
GACAG 3054605 4.957172 208.28545 21
ACAGA 3213365 4.2779913 170.68446 22
TCACA 2165265 4.1153245 79.473816 31
GTCAC 1750390 4.0553374 98.67782 30
TCTCG 1241720 3.9100246 143.13518 42
CACTT 1487540 3.8426018 107.87976 34
TAGAT 1999795 3.8409977 39.698975 22
CTCGT 1196150 3.7665305 143.08679 43
ACTTG 1447295 3.559297 102.02571 35
AGTCA 1938230 3.507105 77.08549 29
AAGAT 2466350 3.485376 79.74774 22
CTTGA 1390950 3.4207292 102.31495 36
ATCTC 1239020 3.2006269 117.460495 41
CGTAT 1224675 3.0118134 112.00504 45
ACACT 1504885 2.8601997 79.14229 33
TCGTA 1161730 2.8570144 112.17584 44
TCCGA 1164160 2.6971483 41.722397 17
TGAAT 1395610 2.680542 85.58023 38
GTATG 1124285 2.6322923 106.9948 46
AATCT 1225935 2.4732926 90.04156 40
TGAAG 1424685 2.4542162 92.5586 20
ACAGT 1287655 2.329931 42.71559 4
AGTTC 932740 2.2938645 57.796017 14
GAATC 1265770 2.2903314 80.11123 39
GTCCG 746180 2.1073413 25.372398 9
GAGAT 1129720 1.9460986 31.34497 20
TGGCT 645155 1.9340619 71.57922 8
GTAGA 1108825 1.910104 24.809048 20
AGTCC 744375 1.7245823 20.432487 8
CAGTG 781675 1.7241254 48.667995 5
TTCCG 540265 1.7012285 71.11802 16
GTTCC 538175 1.6946476 73.3378 15
GTTCT 499520 1.6696438 14.9394655 1
TCTAC 617340 1.5947077 22.683128 3
GTGGC 588405 1.5820423 64.72812 7
TTCTA 552255 1.5142983 22.868752 2
TACAG 818795 1.4815582 15.741571 5
GGCTA 655590 1.4460223 52.777943 9
CTACA 759500 1.4435134 16.419155 4
GTAGT 602810 1.4113611 41.563522 5
CGATC 588225 1.362811 18.807833 15
CCGAC 577130 1.2596525 18.397425 11
GGTAG 591840 1.2427891 37.31211 4
AGTGG 591450 1.2419702 50.071823 6
AGTAG 711450 1.2255707 32.660778 7
TAGTA 634050 1.2178171 36.320004 6
TTCAC 470675 1.2158438 53.38309 1
TTAGA 629600 1.20927 19.690664 21
TTGTA 440835 1.1507958 32.833527 13
CGACG 548515 1.1397669 17.507662 12
AAGTT 585525 1.1246153 45.055344 13
GTTAG 479915 1.1236267 21.722437 20
TTTGT 309415 1.0978103 26.106153 12
GACGA 676120 1.0972427 13.722955 13
TAAGT 556540 1.0689441 45.28515 12
CTAAG 574600 1.0397027 42.80178 11
GCTAA 570365 1.0320396 43.273933 10
ACGAT 568800 1.029208 15.064242 14
CACAG 582940 0.99369323 35.91634 3
GATCA 548940 0.99327236 14.237561 16
TGTAG 422420 0.98901343 15.485679 20
TGAGG 466330 0.97923404 34.074 1
TCAGT 363495 0.8939343 16.68059 16
CCGAG 427385 0.8880693 31.066378 18
CGAGA 528510 0.8576936 24.509258 19
GAGGT 408390 0.85756725 34.934486 2
TGTTA 324710 0.8476526 17.3009 20
TGAGA 487505 0.8397946 10.95335 20
TGCTG 270275 0.8102373 17.981886 16
AGAAC 583825 0.7772532 12.804218 34
GTTTG 241770 0.76934975 23.542162 11
GATTG 327635 0.7670929 23.32536 11
GTGCT 250735 0.7516597 18.385399 15
AGGTA 416520 0.71751314 28.556425 3
TGTAT 273780 0.71470034 29.775454 14
GCTGT 237635 0.7123883 18.175379 17
ATTGT 271740 0.7093749 25.663464 12
TAGTT 265795 0.6938555 18.348509 9
TTTGA 259315 0.6769395 17.55398 9
ACAAG 497355 0.66213465 20.967684 22
CAAGA 484075 0.64445484 20.99555 23
GTGTT 201435 0.64099747 21.08519 19
AGAGA 496105 0.6287875 12.51464 32
GAGTT 267430 0.62613475 8.107926 3
TGTGC 204415 0.6128005 18.65878 14
GAACA 449215 0.5980453 12.835104 35
CTAGA 328030 0.5935496 5.5099163 23
TTGAG 250530 0.58656675 13.829283 19
AGATT 292545 0.5618899 17.023085 10
TTGTG 173770 0.5529631 20.025345 13
ATAGT 287485 0.5521713 20.480375 17
TATAG 281360 0.54040694 21.136847 16
TGAAA 377495 0.53346515 10.275068 11
GACAA 399470 0.53181916 21.537691 21
CTGTT 157140 0.5252399 16.132206 18
GTATA 269785 0.5181749 21.437729 15
GATAG 299275 0.51554245 8.008266 15
AGTTT 193765 0.5058219 18.333693 10
GCACT 215665 0.49965683 8.494962 6
GCACC 224875 0.4908155 14.646439 3
AGTGT 209565 0.4906552 15.765867 18
GAGAA 386555 0.48993853 12.343723 33
ATTTG 185485 0.48420697 17.396505 8
AACAC 345950 0.48377421 13.491791 36
TGACT 196265 0.4826697 6.6521254 20
CTGTA 194535 0.4784152 6.621057 19
CTCAG 203405 0.47125262 6.6030006 4
CATTT 168605 0.46231952 18.594774 7
TAGCA 254680 0.4608274 18.030941 1
TTCAG 185465 0.45610952 7.0670114 9
TGCAC 189820 0.43977863 8.195183 5
CAGTT 177770 0.4371854 7.296544 6
AGAGT 251855 0.43385497 6.0600133 2
ATAGG 248910 0.4287818 11.514827 16
ACTGT 169625 0.4171546 5.7579074 18
CCATT 160625 0.41492525 17.524527 6
ACTTT 148900 0.40828785 11.426478 16
CTTTG 116950 0.39090487 13.087263 17
AGGCT 169485 0.37382978 6.2574906 18
AGGTT 158300 0.37062833 7.652323 10
AAATC 243730 0.36178735 10.728564 13
GCTCA 155460 0.36017272 6.7731075 3
TAGGT 148970 0.34878394 7.2841 9
ATTCT 126335 0.34641406 10.489623 1
GAAAT 244240 0.34515303 10.160764 12
TTGGA 146540 0.3430946 5.612538 4
TCCGT 107070 0.3371504 10.205595 17
AGCCT 141515 0.32786468 9.993809 2
CGTAG 147810 0.32602167 6.51537 19
CAGGA 196860 0.31947467 7.043819 12
CACCA 175120 0.313555 11.848494 4
AGTGC 141890 0.31296402 7.763374 3
GTTGT 97255 0.30948052 8.767606 12
GGCTC 109485 0.30920458 7.4856043 2
CAAGT 167685 0.30341548 6.6021566 3
CCTAT 116560 0.30109692 11.130931 4
TAGCC 129885 0.30092007 11.19819 1
GTGCA 135795 0.29952043 7.9475465 4
ACCAT 156070 0.2966282 12.611707 5
TCGGG 110210 0.29632124 6.851274 27
GGGAG 156960 0.29560897 5.0300837 29
CCAGG 141450 0.29392093 6.698865 11
AACTT 143455 0.2894168 8.182394 15
GGTTG 100650 0.287257 8.981288 11
CTATC 110810 0.2862435 11.172551 5
ACTAC 148365 0.28198403 6.974029 8
GCCTA 120205 0.27849326 10.129501 3
GCCAG 132700 0.2757392 5.144554 6
CGGGA 137795 0.272591 5.150865 28
GTTCA 109325 0.2688603 7.008354 8
ATTGG 113910 0.26669788 6.34124 3
GTAGG 125075 0.2626417 6.677293 8
GGATA 152095 0.2620046 7.523161 14
TAGGC 117370 0.25888073 7.590456 17
TTCCA 98950 0.25560686 6.1584525 12
CAGAA 190410 0.2534951 5.196174 12
CACTA 133240 0.25323728 6.9543233 7
AATCA 170530 0.2531309 10.060629 14
AGTTG 106360 0.24902102 5.76156 9
TCCAT 95990 0.24796064 6.2426567 13
TATGC 99340 0.24430445 7.356367 46
CACAA 174535 0.2440686 5.1655135 32
AGGAT 141545 0.24383079 7.4004984 13
TGCCA 101295 0.2346822 5.4031334 5
TCAGC 101145 0.23433468 6.7282715 10
ATTCC 89385 0.23089863 6.1454253 11
CCGTA 99345 0.23016441 6.8708034 18
AGTTA 119510 0.22954234 7.046498 19
TCAAG 126790 0.22941855 6.597969 2
AATCC 120165 0.22838682 5.825807 8
CTGCC 74495 0.2209876 7.022233 4
ATTGA 113580 0.21815264 5.208728 36
AGCGT 98535 0.21733673 6.95019 2
TTCAA 106710 0.2152847 8.3117075 1
GCGTA 96210 0.21220854 6.9511967 3
AGCTG 96155 0.21208721 5.781656 2
GTAAT 109040 0.20943266 7.9791946 6
ATCCA 109245 0.20763215 5.8235474 9
TAGCG 92835 0.20476435 7.202387 1
GTATC 81935 0.20150073 7.780926 5
AAGCT 111255 0.20130895 5.0022845 1
GCAGG 99080 0.1960036 5.636773 13
GCTGC 69050 0.19500914 6.865833 3
TAATC 92105 0.18581949 6.2057524 7
AAGTA 124180 0.17548764 5.346233 4
AATTG 91180 0.17512903 5.214904 35
AGTAA 121940 0.17232212 5.285547 5
CAATT 82100 0.16563466 5.5114994 34